Precision gene targeting to improve cell line performance in therapeutic production
Our Goal
To become a leading provider of custom host cell line engineering services.
Our Offering
Help improve selection, yield and timelines for biomanufacturers by offering a definitive platform for routine modification of host genomes whilst maintaining the stability of the cell line.
Our Technology
Horizon Discovery provides custom cell line engineering services to pharmaceutical and biotechnology companies worldwide. Utilizing proprietary GENESIS™ technology (rAAV gene targeting platform) and custom X-MAN™ (engineered isogenic cell lines), Horizon can address your quality control and performance requirements for the next generation of producer cell lines. These genetically optimized cell lines will improve the work-flows and efficiencies of current biotherapeutic production.
Figure 1: schematic of rAAV vector design for integration of a point mutation
The key features of rAAV GENESIS™ related to the field of bioprocessing are:
1.Recombinant AAV vectors preferentially up-regulate the homologous recombination pathway (over non homologous end joining) in somatic mammalian cell lines, enabling site specific gene knock-in or knock-out of the host genome without any off-target effects
2.Simple and rapid method to perform any genome alteration in mammalian cells with high targeting efficiencies between 1-10 %
3.QC processes tailored to regulations surrounding therapeutic production including;
- Human packaging cell lines derived from HEK 293 tested for the following human viruses: Adenovirus type 5, Hepatitis A, Herpes Simplex Virus 1, Human Immunodeficiency Virus 1, Rhinovirus, Reovirus type3 and Vaccine virus.
- Engineered host cell line tested for wild-type AAV (PCR & Plaque assay)
For more information on rAAV GENESIS™ click here
Cell Optimization Service
Our custom host cell line engineering service delivers a fully engineered and characterized host cell line, and allows you to modify any gene at any locus in your proprietary host cell line.
- Enables flexible gene engineering approaches such as ; gene ablation; gene defunctionalisation, gene knock-in, inducible gene expression; point mutations; fusion tags
- Selected applications: knockout of glutamine synthetase, knockout of confounding glycosylation genes, site-specific integration
- Zero off-target effects guaranteed
- Proven technology in generating hundreds of isogenic ‘knock-in’ and ‘knock-out’ disease models
FOR MORE INFORMATION:
Please email: info@horizondiscovery.com
Please dial: +44 (0) 1223 655 583 (UK) +1 617 418 4190 (USA)
BioProduction Downloads
Please click on the following links to download relevant materials
rAAV mediated genomic cell line optimization (PDF)
Click here to download
General literature of rAAV-mediated gene targeting (WinZip file)
Click here to download
Literature related to rAAV & Bioproduction (WinZip file)
Click here to download
Bibliography (PDF)
Click here to download
